My answer is a pragmatic one.
First decide on a local hill where it is easy to obtain your powered top speed by rolling down it.
Bring your wrenches for adjusting toe in, and some fore knowledge of how much to turn your adjuster to achieve a one m.m. change in toe.
Start out down the hill with the zero toe setting. If you can achieve your powered top speed whilst in neutral- so much the better. Note the speed achieved before worry sets in and note if it is due to wanderlust or a tendency to dartiness. The former implies not enough toe in, whilst the latter implies too much.
If it is hard to discriminate, go by 1m.m. increments at first, and end up using the least amount of toe in needed to achieve best directional control and least alarming characteristics without detracting from top speed maintained/attained depending on the slope of your hill.
Some compromise between speed attained and best feel may be needed- pick your poison.
At least that was the way that this technique worked for me when I set my HMV Freeway toe in setting. That was when it still had atrocious and dangerous steering and handling due to improperly engineered built in steering control and suspension induced geometries
I would go down my hill, and not glance upon the speedometer until I began to realistically worry about loosing control/ just felt uncomfortable enough not to wish to push my speed further, and also note whether that point was accompanied by more of a wander, or a tendency for the car to more suddenly wig wag, and then adjust my toe in by the tinyest creak of the wrench.
The balance of terrors in my case was achieved at only about 1/100 ths of an inch of toe in.
The Isetta's steering cannot be as flawed, so such a small increment is not likely to be discriminated in your case.